This study attempted to investigate the effect of over-learning and off-line different periods on stabilization-based consolidation process and proactive interference in explicit motor memory. Previous experiences can affect future performance. However, it is not clear how much exercise is needed to stimulate these effects, and what is the best offline period between workouts to prevent interference? In this research, the performance of 40 female students, who were between 20-30 years old and had been called to attend in the test voluntarily, was studied in two offline Periods of 5 minutes and 24 hours between the first (forehand) and the second (backhand) strokes. Data were analyzed using a variance analysis test with repeated measurement tests and a Tukey follow-up test. Two main findings emerged from the study. Firstly, it was determined that offline period (5 minutes and 24 hours) are not effective on the learning of the second task. The second finding was that overlearning performed consolidation in both of the offline Periods (24 hours and 5 minutes) and the learning of backhand stroke became resistant to anterograde interference. These results suggest that learning can play an important role in explicit motor memory, but offline period cannot make learning resistant to interference.