The present study aimed to investigate the association between academic self-actualization, self-regulation, and classroom socio-psychological climate with students' academic motivation with the mediation of achievement emotions in high school students. The statistical population of this cross-sectional correlational study consisted of all high school students in Tehran in 2019, with 485 students selected using multistage cluster sampling. The research instruments included the Academic Motivation Scale (AMS), Self-Actualization Scale (SAS), Motivated Strategies for Learning Questionnaire (MSLQ), Classroom Socio-psychological climate, and Achievement Emotions Questionnaire (AEQ). Data were analyzed by structural equation modeling (SEM) in SPSS and Lisrel software. The results showed that academic self-actualization, achievement emotions, and classroom socio-psychological climate had a positive association with academic motivation in the high school students (p<0.01). Moreover, there was a significant association between self-regulation and academic motivation among the students (p<0.01). The findings indicated that academic self-actualization, self-regulation, and classroom socio-psychological climate had an effect on students' academic motivation, with achievement emotions acting as a mediator (p<0.01).